vsv g envelope protein (Addgene inc)
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vsv g envelope protein
Vsv G Envelope Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g/pCMV-VSV-G+(Plasmid+%238454)/pm41922576-124-21-25
Average 96 stars, based on 3107 article reviews
Vsv G Envelope Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g/pCMV-VSV-G+(Plasmid+%238454)/pm41922576-124-21-25
Average 96 stars, based on 3107 article reviews
vsv g envelope protein - by Bioz Stars,
2026-10
96/100 stars
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Produced:Article Title: Cell size-dependent mRNA transcription drives proteome remodeling Article Snippet: An shRNA that targets the 3′-UTR of human CCND1 mRNA (sequence: 5′-GCCAGGATGATAAGTTCCTTTC-3′) was cloned into the XhoI - MluI sites of the pINDUCER11 vector (Addgene; 44363), as previously described. .. Lentiviral particles were produced in 293T cells by co-transfection of either pINDUCER11 or pINDUCER20 with the packaging plasmid pCMV-dR8.2 dvpr (Addgene; 8455) and the Plasmid Preparation:Article Title: Cell size-dependent mRNA transcription drives proteome remodeling Article Snippet: An shRNA that targets the 3′-UTR of human CCND1 mRNA (sequence: 5′-GCCAGGATGATAAGTTCCTTTC-3′) was cloned into the XhoI - MluI sites of the pINDUCER11 vector (Addgene; 44363), as previously described. .. Lentiviral particles were produced in 293T cells by co-transfection of either pINDUCER11 or pINDUCER20 with the packaging plasmid pCMV-dR8.2 dvpr (Addgene; 8455) and the Article Title: USP30-mediated Deubiquitination of Hexokinase 2 controls the metabolic fate of glucose and tumor progression. Article Snippet: The resulting plasmids were verified by restriction enzyme digestion and DNA sequencing (BGI). .. Lentivirus production and stable cell lines Lentivirus packing was performed by co-transfecting HEK293T cells with the lentiviral packaging plasmid psPAX2 (Addgene, #12260), the Article Title: Identification and inhibition of the Cyclin D Rb-docking interface that drives cell division Article Snippet: To generate the cyclin variants, we performed site-directed mutagenesis using the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, Cat. No. 200521). .. CCND1 cDNAs were subsequently transferred into pINDUCER20 (Addgene; 44012) with the LR clonase reaction (Invitrogen, cat. No. 11791-020). shRNA that targets the 3’-UTR of CCND1 mRNA was subcloned in XhoI – MluI sites in pINDUCER11 (Addgene; 44363), as it was described before . pINDUCER11 and pINDUCER20 lentiviral constructs were packaged in 293T cells by transient transfection, in combination with the Article Title: Deficiency of PTEN Confers Hypersensitivity to Fatty Acid-Mediated ER Stress in Transformed Hepatocytes Article Snippet: DNA transfection : Cells were first plated in a fresh medium at a confluency of 60–70% for 24 h. Then, a plasmid of interest was resuspended in Opti-MEM medium (Thermo Fisher Scientific, 31985062; Waltham, MA, USA) and incubated for 10 min, then thoroughly mixed with X-tremeGENETM 360 Transfection Reagent (Millipore Sigma, X-tremeGENE; St. Louis, MO, USA) at ratio of 1 μg to 3 μL, then incubated for 20 min. .. This mixture was positioned dropwise on the plated cells, which were cultured thereafter for an additional 24–72 h, with fresh medium replacement every 24 h. Lentiviral transduction : HEK293T cells were transfected with the lentiviral transfer plasmid of interest, along with the lentiviral packaging plasmid pCMV-dR8.2 dvpr (addgene #8455) and the Article Title: Tumor-immune-neural circuit disrupts energy homeostasis in cancer cachexia. Article Snippet: Briefly, lentivirus vector was co-transfected into HEK-293T cells with packaging plasmid psPAX2 (#12260; Addgene) .. REAGENT or RESOURCE SOURCE IDENTIFIER RAW264.7 ATCC TIB-71 HEK-293 ATCC CRL-3216 Software and algorithms FlowJo TreeStar https://www.flowjo.com/solutions/flowjo IBM SPSS Statistics 20 IBM https://www.ibm.com/products/spss- statistics ImageJ NIH https://imagej.nih.gov/ij/ Prism 10 GraphPad https://www.graphpad.com R v4.2.3 N/A https://www.r-project.org/ Cancer Cell 44, 1–16.e1–e6, May 11, 2026 e3 and Article Title: Therapeutic targeting of YOD1 disrupts the PAX-FOXO1/N-Myc feedback loop in rhabdomyosarcoma Article Snippet: .. The packaging plasmid pRΔ8.9 and Article Title: Deficiency of PTEN Confers Hypersensitivity to Fatty Acid-Mediated ER Stress in Transformed Hepatocytes Article Snippet: DNA transfection: Cells were first plated in a fresh medium at a confluency of 60–70% for 24 h. Then, a plasmid of interest was resuspended in Opti-MEM medium (Thermo Fisher Scientific, 31985062; Waltham, MA, USA) and incubated for 10 min, then thoroughly mixed with X-tremeGENETM 360 Transfection Reagent (Millipore Sigma, X-tremeGENE; St. Louis, MO, USA) at ratio of 1 μg to 3 μL, then incubated for 20 min. .. This mixture was positioned dropwise on the plated cells, which were cultured thereafter for an additional 24–72 h, with fresh medium replacement every 24 h. Lentiviral transduction: HEK293T cells were transfected with the lentiviral transfer plasmid of interest, along with the lentiviral packaging plasmid pCMV-dR8.2 dvpr (addgene #8455) and the Article Title: USP30-mediated Deubiquitination of Hexokinase 2 controls the metabolic fate of glucose and tumor progression Article Snippet: The resulting plasmids were verified by restriction enzyme digestion and DNA sequencing (BGI). .. Lentivirus packing was performed by co-transfecting HEK293T cells with the lentiviral packaging plasmid psPAX2 (Addgene, #12260), the Transfection:Article Title: Cell size-dependent mRNA transcription drives proteome remodeling Article Snippet: An shRNA that targets the 3′-UTR of human CCND1 mRNA (sequence: 5′-GCCAGGATGATAAGTTCCTTTC-3′) was cloned into the XhoI - MluI sites of the pINDUCER11 vector (Addgene; 44363), as previously described. .. Lentiviral particles were produced in 293T cells by co-transfection of either pINDUCER11 or pINDUCER20 with the packaging plasmid pCMV-dR8.2 dvpr (Addgene; 8455) and the Article Title: Identification and inhibition of the Cyclin D Rb-docking interface that drives cell division Article Snippet: To generate the cyclin variants, we performed site-directed mutagenesis using the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, Cat. No. 200521). .. CCND1 cDNAs were subsequently transferred into pINDUCER20 (Addgene; 44012) with the LR clonase reaction (Invitrogen, cat. No. 11791-020). shRNA that targets the 3’-UTR of CCND1 mRNA was subcloned in XhoI – MluI sites in pINDUCER11 (Addgene; 44363), as it was described before . pINDUCER11 and pINDUCER20 lentiviral constructs were packaged in 293T cells by transient transfection, in combination with the Article Title: Deficiency of PTEN Confers Hypersensitivity to Fatty Acid-Mediated ER Stress in Transformed Hepatocytes Article Snippet: DNA transfection : Cells were first plated in a fresh medium at a confluency of 60–70% for 24 h. Then, a plasmid of interest was resuspended in Opti-MEM medium (Thermo Fisher Scientific, 31985062; Waltham, MA, USA) and incubated for 10 min, then thoroughly mixed with X-tremeGENETM 360 Transfection Reagent (Millipore Sigma, X-tremeGENE; St. Louis, MO, USA) at ratio of 1 μg to 3 μL, then incubated for 20 min. .. This mixture was positioned dropwise on the plated cells, which were cultured thereafter for an additional 24–72 h, with fresh medium replacement every 24 h. Lentiviral transduction : HEK293T cells were transfected with the lentiviral transfer plasmid of interest, along with the lentiviral packaging plasmid pCMV-dR8.2 dvpr (addgene #8455) and the Article Title: Deficiency of PTEN Confers Hypersensitivity to Fatty Acid-Mediated ER Stress in Transformed Hepatocytes Article Snippet: DNA transfection: Cells were first plated in a fresh medium at a confluency of 60–70% for 24 h. Then, a plasmid of interest was resuspended in Opti-MEM medium (Thermo Fisher Scientific, 31985062; Waltham, MA, USA) and incubated for 10 min, then thoroughly mixed with X-tremeGENETM 360 Transfection Reagent (Millipore Sigma, X-tremeGENE; St. Louis, MO, USA) at ratio of 1 μg to 3 μL, then incubated for 20 min. .. This mixture was positioned dropwise on the plated cells, which were cultured thereafter for an additional 24–72 h, with fresh medium replacement every 24 h. Lentiviral transduction: HEK293T cells were transfected with the lentiviral transfer plasmid of interest, along with the lentiviral packaging plasmid pCMV-dR8.2 dvpr (addgene #8455) and the Stable Transfection:Article Title: USP30-mediated Deubiquitination of Hexokinase 2 controls the metabolic fate of glucose and tumor progression. Article Snippet: The resulting plasmids were verified by restriction enzyme digestion and DNA sequencing (BGI). .. Lentivirus production and stable cell lines Lentivirus packing was performed by co-transfecting HEK293T cells with the lentiviral packaging plasmid psPAX2 (Addgene, #12260), the shRNA:Article Title: Identification and inhibition of the Cyclin D Rb-docking interface that drives cell division Article Snippet: To generate the cyclin variants, we performed site-directed mutagenesis using the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, Cat. No. 200521). .. CCND1 cDNAs were subsequently transferred into pINDUCER20 (Addgene; 44012) with the LR clonase reaction (Invitrogen, cat. No. 11791-020). shRNA that targets the 3’-UTR of CCND1 mRNA was subcloned in XhoI – MluI sites in pINDUCER11 (Addgene; 44363), as it was described before . pINDUCER11 and pINDUCER20 lentiviral constructs were packaged in 293T cells by transient transfection, in combination with the Construct:Article Title: Identification and inhibition of the Cyclin D Rb-docking interface that drives cell division Article Snippet: To generate the cyclin variants, we performed site-directed mutagenesis using the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, Cat. No. 200521). .. CCND1 cDNAs were subsequently transferred into pINDUCER20 (Addgene; 44012) with the LR clonase reaction (Invitrogen, cat. No. 11791-020). shRNA that targets the 3’-UTR of CCND1 mRNA was subcloned in XhoI – MluI sites in pINDUCER11 (Addgene; 44363), as it was described before . pINDUCER11 and pINDUCER20 lentiviral constructs were packaged in 293T cells by transient transfection, in combination with the Cell Culture:Article Title: Deficiency of PTEN Confers Hypersensitivity to Fatty Acid-Mediated ER Stress in Transformed Hepatocytes Article Snippet: DNA transfection : Cells were first plated in a fresh medium at a confluency of 60–70% for 24 h. Then, a plasmid of interest was resuspended in Opti-MEM medium (Thermo Fisher Scientific, 31985062; Waltham, MA, USA) and incubated for 10 min, then thoroughly mixed with X-tremeGENETM 360 Transfection Reagent (Millipore Sigma, X-tremeGENE; St. Louis, MO, USA) at ratio of 1 μg to 3 μL, then incubated for 20 min. .. This mixture was positioned dropwise on the plated cells, which were cultured thereafter for an additional 24–72 h, with fresh medium replacement every 24 h. Lentiviral transduction : HEK293T cells were transfected with the lentiviral transfer plasmid of interest, along with the lentiviral packaging plasmid pCMV-dR8.2 dvpr (addgene #8455) and the Article Title: Deficiency of PTEN Confers Hypersensitivity to Fatty Acid-Mediated ER Stress in Transformed Hepatocytes Article Snippet: DNA transfection: Cells were first plated in a fresh medium at a confluency of 60–70% for 24 h. Then, a plasmid of interest was resuspended in Opti-MEM medium (Thermo Fisher Scientific, 31985062; Waltham, MA, USA) and incubated for 10 min, then thoroughly mixed with X-tremeGENETM 360 Transfection Reagent (Millipore Sigma, X-tremeGENE; St. Louis, MO, USA) at ratio of 1 μg to 3 μL, then incubated for 20 min. .. This mixture was positioned dropwise on the plated cells, which were cultured thereafter for an additional 24–72 h, with fresh medium replacement every 24 h. Lentiviral transduction: HEK293T cells were transfected with the lentiviral transfer plasmid of interest, along with the lentiviral packaging plasmid pCMV-dR8.2 dvpr (addgene #8455) and the Transduction:Article Title: Deficiency of PTEN Confers Hypersensitivity to Fatty Acid-Mediated ER Stress in Transformed Hepatocytes Article Snippet: DNA transfection : Cells were first plated in a fresh medium at a confluency of 60–70% for 24 h. Then, a plasmid of interest was resuspended in Opti-MEM medium (Thermo Fisher Scientific, 31985062; Waltham, MA, USA) and incubated for 10 min, then thoroughly mixed with X-tremeGENETM 360 Transfection Reagent (Millipore Sigma, X-tremeGENE; St. Louis, MO, USA) at ratio of 1 μg to 3 μL, then incubated for 20 min. .. This mixture was positioned dropwise on the plated cells, which were cultured thereafter for an additional 24–72 h, with fresh medium replacement every 24 h. Lentiviral transduction : HEK293T cells were transfected with the lentiviral transfer plasmid of interest, along with the lentiviral packaging plasmid pCMV-dR8.2 dvpr (addgene #8455) and the Article Title: Deficiency of PTEN Confers Hypersensitivity to Fatty Acid-Mediated ER Stress in Transformed Hepatocytes Article Snippet: DNA transfection: Cells were first plated in a fresh medium at a confluency of 60–70% for 24 h. Then, a plasmid of interest was resuspended in Opti-MEM medium (Thermo Fisher Scientific, 31985062; Waltham, MA, USA) and incubated for 10 min, then thoroughly mixed with X-tremeGENETM 360 Transfection Reagent (Millipore Sigma, X-tremeGENE; St. Louis, MO, USA) at ratio of 1 μg to 3 μL, then incubated for 20 min. .. This mixture was positioned dropwise on the plated cells, which were cultured thereafter for an additional 24–72 h, with fresh medium replacement every 24 h. Lentiviral transduction: HEK293T cells were transfected with the lentiviral transfer plasmid of interest, along with the lentiviral packaging plasmid pCMV-dR8.2 dvpr (addgene #8455) and the Software:Article Title: Tumor-immune-neural circuit disrupts energy homeostasis in cancer cachexia. Article Snippet: Briefly, lentivirus vector was co-transfected into HEK-293T cells with packaging plasmid psPAX2 (#12260; Addgene) .. REAGENT or RESOURCE SOURCE IDENTIFIER RAW264.7 ATCC TIB-71 HEK-293 ATCC CRL-3216 Software and algorithms FlowJo TreeStar https://www.flowjo.com/solutions/flowjo IBM SPSS Statistics 20 IBM https://www.ibm.com/products/spss- statistics ImageJ NIH https://imagej.nih.gov/ij/ Prism 10 GraphPad https://www.graphpad.com R v4.2.3 N/A https://www.r-project.org/ Cancer Cell 44, 1–16.e1–e6, May 11, 2026 e3 and |